Influence of Zn Stresses on Growth and Physiology in Khus-khus (vetiveria Zizanoides Nash.) and Its Essential Sesquiterpene Oil(s), in Relation to Roots Diameter Circumferential Positions

Khus-Khus (Vetiveria zizanoides Nash.) plants slips of 12.5-15 cms. length, were raised in contoled glass house conditions in different 0 –0. 5 and 1.0 Zn mg/l applications. Total monoterpene oil(s) maximum (0.21 %) was obtained at 0.250 Zn mg/l application at 4-5 inner one circumferential position of roots. At middle 4 th. position of leaf, net photosynthetic rate, and contents of chlorophyll were affected. The maximum peroxidase activity was obtained at middle position of leaf and roots circumferances area, with the maximum production of biomolecule of khusimol and khusinol at 250 mg Zn/L. The maximum of monoterpene oil(s) (0.21%) was also found at middle position of developed roots . However, the relative contents of Khusimol and and Khusinol, which varied at different circumferanceal area of positions. As a result of different root developmental positions, the contents of Fe, Mn, Zn, and Cu were smaller in quantity in Vetiver. Their maximum contents were observed at middle positions of developed root‘s. For commercial exploitation of the total monoterpene oil(s), the midle circumferential position roots are more iportant for hydro distilation of Khu-Khus oil.